At embryonic day (E) 7.5, CEP164-KO embryos showed no obvious morphological abnormalities; however, at E9.5 and E10.5, they exhibited holoprosencephaly, cardiac looping defects, an edematous pericardial sac, and a truncated posterior trunk (Fig 1A). The gene discussed is CEP164; the disease is holoprosencephaly.