GRHL3 and spina bifida: Whereas transgenic expression of Grhl3 has been reported25, utilising a BAC to restore Grhl3 expression and rescue spina bifida-like defects in a hypomorphic Grhl3 model (the curly-tail mouse), this study did not report any over-expression-related phenotypic abnormalities in these mice, nor were over-expression studies performed using the BAC construct in WT mice.