To further confirm the effects of miR-221-3p on TWIST2-induced EMT in cervical cancer cell lines, SiHa-tw2 cells were transfected with miR-221-3p inhibitor, SiHa-shtw2 cells were transfected with miR-221-3p mimic, and HeLa cells were transfected with TWIST2 expression vector with/without miR-221-3p mimic or TWIST2 siRNA with/without miR-221-3p inhibitor. This evidence concerns the gene TWIST2 and cervical cancer.