We also compared the response of the established EGFR mutant and ALK-translocated patient-derived NSCLC cell lines to EGFR or ALK inhibition growing in the presence of TCM media without EGF and insulin (TCM, -E, -I media; Figures 2I–2K, orange curves) to that of the same cells grown in their native media, R10 (Figures 2I–2K, blue curves), in the presence of irradiated feeder fibroblasts (i.e., mixed cultures). The gene discussed is ALK; the disease is non-small cell lung carcinoma.