INS and non-small cell lung carcinoma: We also compared the response of the established EGFR mutant and ALK-translocated patient-derived NSCLC cell lines to EGFR or ALK inhibition growing in the presence of TCM media without EGF and insulin (TCM, -E, -I media; Figures 2I–2K, orange curves) to that of the same cells grown in their native media, R10 (Figures 2I–2K, blue curves), in the presence of irradiated feeder fibroblasts (i.e., mixed cultures).