The BTG2 expressions were decreased by PTEN knockdown in bladder cancer cells as RT4_shPTEN cells (RT4 cells with PTEN knockdown) exhibited lower BTG2 mRNA expression than RT4_shCtrl cells (RT4 cells with mock knockdown) (Fig. 4B); while T24‐PTEN cells (T24 cells with PTEN overexpression) presented higher BTG2 mRNA expression than T24‐DNA cells (T24 cells with mock overexpression) (Fig. 4C). This evidence concerns the gene PTEN and urinary bladder cancer.