Longer-term interventions based on high-fiber, low-protein diets in obese children suffering from diet-related (“simple”) obesity (SO; 30 days, n = 21) and Prader-Willi syndrome (PWS; 90 days, n = 17) did not significantly affect mean abundances of the Ac pathway in either group (Fig. 5C). Here, ASAH1 is linked to obesity due to melanocortin 4 receptor deficiency.