The aim of our study was to analyze the effects of EGFR signaling in a panel of four human EGFR-expressing gastric cancer cell lines (AGS, Hs746T, LMSU and MKN1) by detailed characterization of the link between the differing motility-focused phenotypic behaviors of the individual cell lines and their specific molecular characteristics. The gene discussed is EGFR; the disease is gastric cancer.