Conversely, no significant reactivity was observed after stimulating the same PBMC samples with FLU or HIV peptides (S3A Fig, CD3+CD8bright: KS test p<0.05; MW test GAD65 (GAD) vs FLU stimulated p = 0.0027, MW test GAD65 vs HIV p = 0.0008; S3B Fig, CD3+CD8dull: KS test p<0.05; MW test GAD65 vs FLU p = 0.0004, MW test GAD65 vs HIV p = 0.0001; S3C Fig, total CD3+CD8+: KS test p<0.05; MW test GAD65 vs T1D FLU p = 0.0004, MW test GAD65 vs HIV p = 0.0001; S3D Fig, CD3-CD8dull: KS test p<0.05; MW test GAD65 vs FLU p = 0.0048, MW test GAD65 vs HIV p = 0.0005). This evidence concerns the gene GAD1 and type 1 diabetes mellitus.