Given that PLX7904 did not induce B-Raf/C-Raf dimers in melanoma lines with N-Ras mutations, but could inhibit ERK activation in these cells and in melanoma lines expressing the dimeric V600E-B-Raf splice variant, suggests that PLX7904 binding may cause enough steric hindrance at the dimer interface to prevent or disrupt Raf dimerisation. This evidence concerns the gene RAF1 and melanoma.