In line with this, PCR array analyses confirmed that loss of OTUD1 led to several molecular features of mesenchymal cells, including the upregulation of key transcriptional inducers such as TWISTs, SNAILs, ZEBs, and other EMT-related targets such as COL1A1, SERPINE1 (Fig. 3h, i; Supplementary Data 3); We next analyzed the influence of OTUD1 overexpression on well-established EMT-related makers genes at 3 days (immediate response) and 12 days (delayed response) post-transduction of OTUD1 expressing lentiviruses in breast cancer MDA-MB-231 (BM) cells (Fig. 3j). Here, OTUD1 is linked to breast cancer.