MA at GV20 was also found to upregulate hippocampal choline transporter 1 (CHT1), vesicular acetylcholine transporter (VAChT), BDNF, and CREB in AD model rats (Table 1) [19], suggesting that manual GV20 stimulation may promote cholinergic neurotransmission as a component of its effects on memory and cognition. Here, BDNF is linked to Alzheimer disease.