Our results firstly showed that antcin-H inhibited the Src/FAK/paxillin and Src/FAK/ERK-c-Fos-C/EBP-β signaling pathways to impair lamellipodium formation and decrease MMP-7 expression, consequently suppressing RCC cell migration and invasion, suggesting that antcin-H might have the potential for treating metastatic RCC. Here, MMP7 is linked to renal cell carcinoma.