The ethanol extract of Hedyotis diffusa Willd [30] could inhibit colon cancer stem cells sphere formation, differentiation in vitro by downregulating the β-catenin and TCF4 mRNA, and inhibiting β-catenin/Wnt pathway and CK20 and CD133 mRNA in colon cancer stem cells, which was time-dose dependent. The gene discussed is PROM1; the disease is malignant colon neoplasm.