Wnt/β-catenin could also affect cancer stem cells differentiation and viability in colon cancer stem cells. Hedyotis diffusa Willd inhibited β-catenin and TCF4 gene transcription in Wnt/β-catenin pathway, suppressed the activity of Wnt/β-catenin, which inhibited the transcription of gene CK20 and gene CD133, and affected CSC differentiation and viability [30]. This evidence concerns the gene TCF4 and malignant colon neoplasm.