Overall, our findings demonstrated that miR-191, miR-222, and miR-224 could play important roles in lipid and cholesterol metabolism and participate in developing disease conditions such as hypercholesterolemia and CVD, by targeting PCSK9 which has a critical role in LDLR degradation and cellular LDL uptake. The gene discussed is LDLR; the disease is familial hypercholesterolemia.