For the selection of the genes to be validated by rtPCR, we prioritized parental genes as the reference biotype, then we selected genes that encode for proteins belonging to cancer-related pathways such as the PI3K-AKT pathway (SGK1 and PIK3CD) or the cell adhesion pathway (MPZ), for kinases (e.g. PINK1, TGFBR1 and TWF1) or for tumor suppressors (FH). The gene discussed is TGFBR1; the disease is cancer.