First, we analysed FOXP3 mRNA in different T-cell subsets in the circulation and tumour mass, and we observed prominent FOXP3 expression in Treg cells in the peripheral blood and CxCa infiltrate, minimal expression in tumour-derived effector T-cells, and a complete lack of expression in naïve T-cell populations from either patients or donors (Figs. 2A and S2a,b). Here, FOXP3 is linked to neoplasm.