WNT5A and obesity due to melanocortin 4 receptor deficiency: To investigate the role of WNT5A and downstream signaling in obesity-associated adipose tissue dysfunction in humans, we used quantitative real-time PCR (qRT-PCR) to evaluate transcript expression of 33 genes involved in WNT/PCP signaling in visceral (omental) and subcutaneous fat obtained from 44 individuals at the time of bariatric surgery.