To further validate the selective inhibition of p38α, we transfected HEK293 cells with either wtp38α or p38α(T185G) and subjected them to simulated ischemia, an experimental setup that tries to recapitulate the low-oxygen and low-pH conditions experienced by cells during ischemia, and we relied on endogenous TAB1 to cause p38α activation. The gene discussed is TAB1; the disease is ischemia.