As TF+ prostate cancer cells demonstrated much higher ability for clonogenic survival and growth, as well as the propensity to form prostaspheres under both differentiating and non-differentiating conditions compared to TF- cells, our next question was whether TF-Ag selection leads to the enrichment of cells with elevated expression of stem cell markers such as CD44 and CD133. Here, CD44 is linked to prostate carcinoma.