In the present work, we employed a method combining biotinylation of cell surface proteins followed by a streptavidin agarose pull-down, lectin affinity chromatography, and LC-MS/MS mass spectrometry analysis to identify cell surface TF-Ag glycoprotein carriers expressed on PC-3 and DU-145 human prostate carcinoma cell lines. Here, TF is linked to prostate carcinoma.