Using the affinity purification of biotinylated cell surface proteins followed by a pull-down with TF-Ag specific peanut agglutinin (PNA) and a shotgun mass spectrometry analysis as well as immunoprecipitation (IP) of individual glycoproteins, and Western blot verification of TF-Ag expression, we unambiguously identified 18 cell surface glycoproteins as TF-Ag carriers expressed on PC-3 and DU-145 metastatic human prostate carcinoma cells. Here, TF is linked to prostate carcinoma.