To evaluate the value of Bcl-3 and HE4 served as biomarkers of renal fibrosis, we tested the levels of serum Bcl-3 and HE4 protein in the UUO model using western blotting due to a lack of reliable commercially available quantitative ELISA kits for mouse Bcl-3 and HE4. The gene discussed is BCL3; the disease is renal fibrosis.