Despite the fact that, in our first experiment, it was not possible to discard any physiological or behavioral effect produced by possible phytoestrogen content in the rat food, the second experiment discards any influence of food on anxiety-like behavior, because nonsignificant differences were found between vehicle-treated rats and rats treated with only tamoxifen (the ERβ antagonist) fed with the same food. This evidence concerns the gene ESR2 and Anxiety.