As shown in Fig. 5a, pcDNA-GAS5 significantly promoted the expression of PTEN mRNA in PANC-1 and BxPC-3 cells, but the level of PTEN mRNA aggressively reduced in PC cells transfected with pcDNA-GAS5 and miR-32-5p mimic. The gene discussed is GAS5; the disease is pachyonychia congenita.