The main new achievement of this study includes: (1) LncRNA GAS5 inhibited the migration and invasion of PC cells; (2) miR-32-5p was increased in PC tissues and cells, and was associated with the migration and invasion of PC cells; (3) we further explicitly confirmed the existence of GAS5/miR-32-5p/PTEN signaling pathway in pancreatic cancer cell metastasis. Here, PTEN is linked to familial pancreatic carcinoma.