In order to study the impact of Gadd45β on liver cancer cells, Gadd45β-expressing adenovirus were transfected into SMMC-7721 and Hep-3B cells, we found that Gadd45β overexpression inhibited the stemness properties of cells, including the downregulation of SOX9 and OCT4 expression, reduction of colony formation. The gene discussed is SOX9; the disease is liver cancer.