Next, we checked the methylation and expression distribution of CLDN11 in CRC cell lines (including HCT116, COLO205, SW620 and HT29) and normal colon cell line (NCM460), observing statistically elevated methylation level in HCT116, and remarkably diminutive methylation level in SW620 compared with in NCM460 (Figure 6a). The gene discussed is CLDN11; the disease is colorectal carcinoma.