From the list of proteins that appeared to be differentially expressed between Huh-7.4 and Huh-7.5 cell lines, GLS (glutaminase kidney isoform, mitochondria), ANXA3 (Annexin A3), ACSL-3 (long chain fatty acyl-coA synthetase 3), and UCHL-1 (Ubiquitin C-terminal hydrolase-L1) proteins have previously been connected to HCC and cancer in general [16, 17]. Here, GLS is linked to hepatocellular carcinoma.