To evaluate the importance of TGFβ1 in CAF-derived exosomes supporting a pre-metastatic niche in vivo, we mixed SKOV-3-Luc cancer cells and CAF, and co-injected them into BALB/c nude mice, with or without an intraperitoneal injection of anti-TGFβ1. The gene discussed is TGFB1; the disease is cancer.