Top performing protein markers consisted of secretory vesicle proteoglycan Serglycin (SRGN); actin filament-binding protein Tropomyosin alpha-3 chain (TPM3); adhesive glycoprotein Thrombospondin-1 (THBS1) and E3 ubiquitin-protein ligase HECT, UBA and WWE domain-containing protein 1 (HUWE1) (Figure 4B), all of which were also detected in lung cancer cell line derived EVs. This evidence concerns the gene TPM3 and lung cancer.