We exploited a multi-disciplinary approach, comprising electron microscopy (EM), Ca2+ imaging, real-time polymerase chain reaction (qRT-PCR), Western blotting, and functional assays to demonstrate that VEGF fails to promote proliferation and in vitro tubulogenesis in BC-ECFCs due to the down-regulation of the underlying repetitive Ca2+ spikes. Here, VEGFA is linked to breast cancer.