To detect anti-HCRTR2 autoantibodies in narcolepsy patients and controls, live HEK293T cells transiently transfected with HCRTR2-GFP were stained with each serum from 88 narcolepsy patients, including 40 post Pandemrix® narcolepsy (PP-N) patients and 48 early onset (EO-N) patients, and 88 healthy controls, including 18 matched post Pandemrix® controls (PP-C) and 70 other controls (O-C), and analyzed with flow cytometry. The gene discussed is HCRTR2; the disease is narcolepsy.