TNF and amyotrophic lateral sclerosis: ALS IgG-treatment induced a rise in TNF-α release (454.31 ± 42.75 pg/ml) compared with control IgG-treated group (160.88 ± 10.37 pg/ml, p < 0.05) as well as with the untreated group (15.15 ± 9.76 pg/ml, p < 0.01), as seen in Figure 4A. The results of the TNF-α gene expression experiments showed no difference between the groups.