The immunohistochemistry results revealed high percentage positivity and increased intensity of MARCH8 staining in 30/35 (86%) ESCC tissues as compared to distant matched non-malignant tissues which either did not show any detectable staining for MARCH8 protein or a very faint staining in lesser proportion of cells in the tissues was observed (Fig. 1c–h, p < 0.001, Table 2). This evidence concerns the gene MARCHF8 and esophageal squamous cell carcinoma.