(A) Flow cytometry results; (B) frequency of Tim-3+ cells among CD4+ and CD8+ cells in circulation of GC patients and HDs; (C) PD-1+ cells on T-cells in circulation of GC patients and HDs; (D) median percentage of PD-1+ Tim-3+ cells among CD4+ and CD8+ in GC patients and HDs; E percentage of Tim-3+ cells in different tissues; F Expression level of PD-1+ for CD8+; G PD-1+ Tim-3+ cells among T-cells. The gene discussed is HAVCR2; the disease is gastric cancer.