In present study, we compared the expression of PD-1+ and Tim-3+ on CD4+/CD8+ T-cells in blood circulation, tumor, and paraneoplastic tissues from patients with GC, and further tested the changes of IFN-γ production on T-cells after in vitro blockade of PD-1+ and/or Tim-3+ pathways. This evidence concerns the gene CD8A and gastric cancer.