To further clarify that PI3K/AKT is responsible for suppression of tumour growth in CMTM5-transfected human HCC cells, we used PI3K/AKT inhibitor LY294002 to examine the effect of AKT inhibition on CMTM5-induced tumour growth suppression (Fig. 4c). This evidence concerns the gene AKT1 and hepatocellular carcinoma.