We investigated the effect of Coptis Chinensis in glioma cells and the associated biological mechanisms, and we further studied the effect of Coptis Chinensis on the relationship between STAT3 and HDAC3 to clarify whether Coptis Chinensis functioned similarly to Sodium Valproate (VPA), which down-regulated the phosphorylation of STAT3 by reducing the expression of HDAC3. This evidence concerns the gene STAT3 and glioma.