LS141 (Liposarcoma) and CHP100 (Ewing Sarcoma) cell lines, on the other hand, have been used extensively and both these cell lines have not been reported to harbor any NF1 mutation/loss [19, 21–24] (also, personal communication with Kanojia D, Cancer Science Institute, Singapore). The gene discussed is NF1; the disease is Ewing sarcoma.