Logistic regression analyses revealed that the PADI4 rs11203366 A>G, rs1886302 T>C, rs1635562 A>T, rs1635564 C>A, rs16825533 A>G, and rs2477137 C>A polymorphisms were not associated with the risk of ESCC. Here, PADI4 is linked to esophageal squamous cell carcinoma.