The results of knockdown experiments showed that knockdown of AFAP1-AS1 could inhibit cell proliferation and migration in lung cancer cells in vitro and suppress lung tumor growth in vivo. Moreover, we found that down-regulation of AFAP1-AS1 could influence the expression of AFAP1 and KRT1 on mRNA and protein level by cis and trans regulatory mechanism. This evidence concerns the gene AFAP1 and lung carcinoma.