We also provided evidence that AFAP1-AS1 regulated the expression of AFAP1 in cis and may interact with KRT1 in trans. Although lncRNA AFAP1-AS1 represented a potentially promising biomarker and provided a new therapeutic tool in treating malignant tumors, more efforts to explore the mechanism of its action in cancer pathogenesis were needed in future research. Here, AFAP1 is linked to cancer.