Antisense lncRNAs are usually reported to act as regulator of gene of the opposite strand [41, 42], but Wu et al. demonstrated that AFAP1-AS1 had limited effect on AFAP1 expression, instead functioning in an AFAP1-independent manner in Barrett’s esophagus and esophageal adenocarcinoma [24]. This evidence concerns the gene AFAP1 and esophageal adenocarcinoma.