In the context of single cell profiling to uncover events such as transcriptional signalling of c-MET associated with early invasive cancer events [7] or with potential in the clinical setting of invasive breast cancer to detect HER2 [22], the robustness and usefulness of a technology such as RNAscope may be hugely beneficial but only if underpinned by the use of controls such as those described in the present study. Here, MET is linked to invasive breast carcinoma.