To determine if p53-induced lipid droplet accumulation is a general function of p53, we assessed the effect of p53 activation on lipid droplet accumulation in three different primary skin fibroblast lines (BJ, HF, 67LR), retinal epithelial cells (RPE), and an osteosarcoma cell line encoding wild type p53 (U2OS). Here, TP53 is linked to osteosarcoma.