Expression of spätzle, a ligand that binds the Toll receptor in the Toll innateimmune response pathway (Xi et al. 2008), wasanalyzed in Ae. aegypti mosquitoes infected with PR DEnV-1 and −2.The highest fold change in expression of this gene resulting from infection witheither virus strain was detected at 1dpi (Fig.1A) with PR DENV-1 having higher levels compared to PR DENV-2, althoughthe level differences were not significant. Here, TLR4 is linked to infection.