In TRYPHAENA study (NCT00976989), 225 patients with HER2-positive breast cancer were recruited and randomized 1:1:1 to receive six neoadjuvant cycles q3w (arm A: 5-fluorouracil, epirubicin, cyclophosphamide [FEC] + trastuzumab + pertuzumab × 3 weeks → docetaxel + trastuzumab + pertuzumab × 3 weeks; arm B: FEC × 3 weeks → docetaxel + trastuzumab + pertuzumab × 3 weeks; arm C: docetaxel + carboplatin + trastuzumab + pertuzumab × 6 weeks). The gene discussed is ERBB2; the disease is breast carcinoma.