Moreover, we pre-treated HT-29 cells with or without Sal-1 antagomir and then infected the cells with Salmonella. As shown in Fig. 3c and d, Salmonella infection increased iNOS expression (Fig. 3c) and NO level (Fig. 3d) in HT-29 cells compared to Mock infection. The gene discussed is NOS2; the disease is Salmonella Infections.