By applying the T3 protocol to macrosections of each tumor and then registering and fusing each macrosection image, we mapped expression of PD-L1, the tumor marker Her2, the immune cell marker CD45, the cell proliferation marker Ki-67, and the endothelial marker CD31 (Fig. 2a–c, Supplementary Video 1, and Supplementary Fig. S4). Here, CD274 is linked to neoplasm.