In addition, our findings confirmed that Miro1 ablation damages beta cell function examined by Cpt2, Acadvl, and Hadhb led to inflammasome activation interfering with mitochondrial secretory function by detecting PDX-1, NeuroD, insulin, Glut2, and urocortin3, suggesting a therapeutic promising of Miro1 for mtROS-related T2D. Here, RHOT1 is linked to type 2 diabetes mellitus.