To test the effects of 90K on E-cadherin levels in the above-mentioned cancer types, HEK293T cells, the colorectal cancer cell line Caco2, the prostate cancer cell lines CWR22Rv-1 and PC3, the lung cancer cell lines H1650 and H358, and the breast cancer cell lines BT474 and MCF7 were treated with 90K conditioned medium (90K/CM). The gene discussed is CDH1; the disease is colorectal cancer.