CD8A and infection: To assess the response to non-gB498-505 HSV-1 epitopes, TG from WT or S1L infected mice at different times post infection were dispersed, stimulated for 6 hrs with B6WT3 fibroblasts infected with HSV-1 gB-null-EGFP virus, and intracellular IFNγ in CD3+CD8+ T cell populations was measured by flow cytometry (Fig 7B).