SMAD2 and neoplasm: In addition, hierarchical clustering of EMT markers according to patients age revealed apparently different character of the tumors diagnosed at age ≤50 years with overexpression of CTNNB1, SMAD2, SMAD3, TCF4 and ZEB1 indicating more aggressive clinical course of the tumor due to predominance of mesenchymal pattern in the youngest males (S2D Fig).