In addition, hierarchical clustering of EMT markers according to patients age revealed apparently different character of the tumors diagnosed at age ≤50 years with overexpression of CTNNB1, SMAD2, SMAD3, TCF4 and ZEB1 indicating more aggressive clinical course of the tumor due to predominance of mesenchymal pattern in the youngest males (S2D Fig). The gene discussed is SMAD3; the disease is neoplasm.