To explore functional consequences of substitutions in human eIF1A associated with uveal melanoma (Martin et al., 2013), we introduced substitutions into the yeast eIF1A NTT corresponding to 7 of the 13 substitutions associated with the disease: K3D, K4D, T6R, T6D, ΔG8, R13P, and G15D (Figure 1C). Here, EIF1 is linked to uveal melanoma.