Induction of γ-GCS and HO-1 is mainly due to transcriptional activation mediated by Nrf2.(13,19) Nrf2 is an important transcription factor, which has been reported to play a key protective role against APAP-induced liver injury.(20) Our results showed that SLH reversed the APAP-induced decrease in Nrf2 expression (Fig. 3B). Here, NFE2L2 is linked to injury.