RT-qPCR analysis of seven additional clinical prostate tumors and their adjacent non-malignant prostate cells confirmed that TAAA-ACTG2 (5/7 cases), GAAA-PTGIS (5/7 cases) are consistently down-regulated (>2-fold change in expression) in tumors compared to adjacent non-malignant tissues, and that TTTTG-TRIB1 (7/7 cases), TTTTTG-PRUNE2 (5/7 cases), TG-PCA3 (5/7 cases) are consistently up-regulated (>2-fold change in expression) in tumors compared to adjacent non-malignant cells (Fig. 3b and Table 1). This evidence concerns the gene PCA3 and prostate neoplasm.